نشریه پژوهش هایی در کشت سلول و بافت کاریوتیک
سال دوم شماره 3 (پاییز 1400)

Studies show that some plant extracts are effective in improving the lifespan of blood cells, although the mechanism of action is not clear in many cases. Accordingly, the present study investigated the effects of Pissum sativum L and Aloe vera extract on cell membrane stability in human red blood cells.

In this experimental laboratory study, blood samples were obtained from healthy individuals and blood samples were divided into control group (treated with normal saline) and 2, 4 and 6 mg/kg of sulfasalazine receiving groups. Samples treated with 2 mg/kg of sulfasalazine were treated with 2, 4 and 6 mg / kg of Pissum sativum L and Aloe vera extract and the erythrocyte membrane stability was calculated by standard methods. Data were analyzed using one-way analysis of variance.

Sulfasalazine significantly reduced the stability of erythrocyte membrane compared to the control group. Treatment of samples with concentrations of 4 and 6 mg / kg Pissum sativum L extract significantly increased membrane stability compared to the groups treated with sulfasalazine. Treatment with 2 and 4 mg / kg of Aloe vera extract did not increase membrane stability and a 6 mg / kg of Aloe vera extract non-significantly increased the membrane stability.

Unlike Aloe vera, Pissum sativum L extract can increase membrane stability of red blood cells in healthy people.

In the second half of the last century, Pseudomonas aeruginosa is considered an important hospital pathogen, according to the US Centers for Disease Control. Pseudomonas aeruginosa is the fifth pathogen among hospital microorganisms, which comprises 10% of hospital-acquired infections, as the purslane plant has a variety of vitamins and minerals in fatty acids and as a fever antiseptic medication and it is used against spasm.
To evaluate the antimicrobial activity of aqueous and ethanolic extracts of Purslane, four standard microbial methods including mixed agar diffusion method, disk diffusion, minimum inhibitory concentration of MIC and minimum killer concentration of MBC using micro dilution method. The results showed that silver nanoparticles obtained from purslane extract with the extract of this plant significantly and significantly reduced the growth of Pseudomonas bacteria, however, treatments of this bacterium treated only with purslane extract. Had not caused the bacterial growth and it can be said that the effect of silver nanoparticles from the plant It was significantly higher than the extracts extracted from this plant and data analysis showed that with increasing concentration of MIC, on the other hand, pH 10 combined with 2 mM nitrate salt yielded the most suitable dimensions for silver nanoparticles, and silver nanoparticles synthesized from purslane had far greater antibacterial effect than the extract extracted from this plant has a good option to fight infection caused by Pseudomonas aeruginosa.

One of the most well-known side effects of anticancer drugs is disruption of spermatogenesis, which in many cases causes infertility. The aim of the present study was to evaluate the expression change of Nrf-2 and Keap-1 genes due to peritoneal injection of busulfan in male Wistar rats.

In this study, 20 two-month-old adult male Wistar albino rats weighing approximately 200-150 g were studied in two groups: control group (healthy) and busulfan group. After this period, the testicular tissue of the mice was isolated and evaluated for the expression of Nrf-2 and Keap-1 genes, as well as changes in testosterone, FSH and LH. Data were analyzed using SPSS software and one way ANOVA statistical test.

Statistical analysis of the results showed that the expression of Nrf-2 and Keap-1 genes in the treatment group (busulfan) decreased compared to the control group, but this decrease was not statistically significant (0.2951 and P = 0.3528). LH and testosterone levels were not significantly different between the control and treated groups (0.1327 and P = 0.0809) but FSH levels were significant between the two groups (P = 0.0189).

The results of this study showed that busulfan can affect the level of FSH, which is effective in spermatogenesis, by causing changes in the expression of keap1 and Nrf-2 genes and cause infertility. Therefore, these genetic and hormonal factors can be used to predict infertility.

Genes play a very important role in the development of the cancer. The aim of this study was to evaluate three very important genes (C-fos, C-jun and P53) in wistar mice stomach tissue cancer cells designed by dimethyl hydrazine.

In this study, forty male Wistar mice, 57 weeks old, were evaluated and divided into four groups of Ten. The control group, the second group received Cinnamaldehyde-free carcinogens, the third group was healthy with the Cinnamaldehyde, and the fourth group received carcinogens with the Cinnamaldehyde at the same time.Then, the gastric tissue of mice was isolated and evaluated for the expression of C-fos, C-Jun and P53 genes. The data was analyzed with One -way Anova using SPSS.

Statistical analysis of the results showed that the expression of C-fos, C-Jun and P53 genes in the carcinogen receptor with the Cinnamaldehyde was increased compared to the control group. This increase was statistically significant for two genes C-fos (P = 0.0146) and P53 (P = 0.0212) and for C-Jun gene (P = 0.01604) this increase was not significant.

The results of this study showed that Cinnamaldehyde can alter the expression of important genes C-fos, C-Jun and P53 that are effective in causing gastric cancer.

Hydatid cyst surgery is the most effective treatment. Hydatid cyst recurrence after surgery and the lack of effective and safe Askvlysydl one of the most important research topics in the treatment of hydatid cysts, which have negative effects on human organs. The aim of this study was to conduct a full investigation on the impact of the different stages of the parasite herbs and ingredients, to treat helminth infections will significantly contribute to a significant reduction in helminth infections. After examining the viability protoscolex 1% eosin staining, protoscolex activities Plumbing chamomile extract diluted concentrations of 5%, 2.50%, 1.25%, 0.66%, 0.33% and in time of 0, 10, 20, 30, 40, 50, 60, 120, h24, h72 were studied. 0/5 chamomile extract concentration in the first two hours protoscolex of hydatid cyst can be completely destroyed. Protoscolices lowest percentage was in the concentration of 0.33% percent in an hour. Dilution of engineering activity increased protoscolex Decreases. In addition to being affordable chamomile extract, in a short time leads to protoscolex within the hydatid cyst. Scolexes property in dilution laying down time is less effective.

This study investigated the molecular and physiological responses of lemon balm seedlings to the manipulation of nutrient solution with zinc oxide nanoparticle (nZnO) at 0, 100, and 300 mgl-1. The augmentation in biomass accumulation and photosynthetic pigments resulted from the nZnO utilization. The exposure of seedlings to nZnO modifed both primary and secondary metabolism in leaves. The nanosupplements upreguated activities of phenylalanine ammonia lyase and catalase enzymes. Moreover, the incease in concentrations of proline, flavonids, and glutathione resulted from the application of nZnO. Furthermore, the nZnO treatments up-regulated the transcription of phenylalanine ammonia-lyase (PAL) and Coumarate: CoA-ligase (4CL) genes, two genes responsible for the production of secondary metabolites. These comparative physiological and molecular investiagtions on plant responses to nZnO and its bulk type may serve as a theoretical basis for function in agricultural and medicinal industries.